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ASTM D7304-16
- Standard Test Method for Determination of Denatonium Ion in Engine Coolant by HPLC
- standard by ASTM International, 05/15/2016
- Publisher: ASTM
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1.1 This test method covers the chemical analysis of engine coolant for denatonium benzoate (DNB) by high-performance liquid chromatography (HPLC). DNB is added to potentially render engine coolant unpalatable to animals and humans. This analytical method was designed for the analysis of DNB and is not valid for any other bittering agents such as denatonium saccharide.