• ASTM E2888-12
Provide PDF Format

Learn More

ASTM E2888-12

  • Standard Practice for Process for Inactivation of Rodent Retrovirus by pH
  • standard by ASTM International, 09/01/2012
  • Publisher: ASTM

$20.00$39.00


This practice assures 5 log10 inactivation of nondefective C-type retroviruses, which are endogenous to murine hybridoma and CHO cells and are potentially present in the production stream of biopharmaceutical processes that use rodent derived cell culture.

1.2 The process parameters specified in this practice consistently assure 5 log10 inactivation of murine retrovirus by adjusting the pH of a process solution after initial affinity capture chromatography purification.

1.3 This practice is applicable to mAb, IgG fusion, or other recombinant proteins produced from rodent cell lines (for example, CHO or murine hybridoma), which do not target retroviral proteins. Additionally, the low pH step is performed on a cell-free intermediate, post initial capture using protein A chromatography.

Related Products

ASTM D5489-07(2013)e1

ASTM D5489-07(2013)e1

Standard Guide for Care Symbols for Care Instructions on Textile Products..

$26.00 $52.80

ASTM D4472-08(2014)

ASTM D4472-08(2014)

Standard Guide for Recordkeeping for Reverse Osmosis and Nanofiltration Systems..

$20.00 $39.00

ASTM D3631-99

ASTM D3631-99

Standard Test Methods for Measuring Surface Atmospheric Pressure..

$26.00 $52.80

ASTM A291-02

ASTM A291-02

Standard Specification for Steel Forgings, Carbon and Alloy, for Pinions, Gears and Shafts for Reduc..

$23.00 $46.80