AWWA PATH56520

The ability of the enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) to discriminate between different E. coli O157:H7 isolates was investigated. Ninety-one sewage, 244 river water, 48 cattle faecal samples and 14 pig faecal samples were screened for E. coli O157:H7 by using the enrichment-IMS-selective agar method and PCR. E. coli O157:H7, carrying the genes coding for Stx2, eaeA and Hly were isolated from 1 sewage sample (0.76% of sewage samples), 8 cattle faecal samples (16.67% of cattle samples) and 2 pig faecal samples (14.28% of pig samples). Total chromosomal DNA was extracted from 31 positive E. coli O157:H7 environmental and faecal isolates which included 2 from sewage, 26 from cattle and 3 from pigs. Repetitive sequence analysis (RSA) of 31 E. coli O157:H7 strains using ERIC-PCR revealed two major clonal populations with two groups in each section. Most cattle E. coli O157:H7 isolates from the same feedlots grouped together (> 70% correlation) although some were scattered among the other groups. The RSA patterns of the porcine E. coli O157:H7 isolates differed quite extensively (30% correlation) while the sewage isolates shared a 50% correlation. The results revealed the highly discriminating features of the RSA ERIC-patterns of E. coli O157:H7 strains of diverse and similar origin. Includes 51 references, table, figures.

Product Details

Edition:

Vol. - No.

Published:

09/22/2002

Number of Pages:

10

File Size:

1 file , 500 KB