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AWWA QTC98336
- The Use of Vortex Flow Filtration, Immunomagnetic Separation and Immunofluorescence for the Improved Recovery and Detection of Cryptosporidium Oocysts from Water
- Conference Proceeding by American Water Works Association, 01/01/1998
- Publisher: AWWA
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Conventional methods for the recovery of Cryptosporidium from water involves filtration of large volumes of water through a cartridge filter, clarification of the sample by density gradients, and detection by immunofluorescent microscopy (IFA). The cartridge filter IFA method is currently being used to collect water samples for an 18 month survey of the drinking water supplies in the United States under the Information Collection Rule (ICR). Various types of filtration are currently being investigated including Gelman Envirochek filter, the polycarbonate membrane filter and the use of vortex flow filtration. Vortex flow filtration and immunomagnetic separation (VFF/IMS) were compared to the standard cartridge filter IFA method by seeding dechlorinated tap water with formalinized Cryptosporidium oocysts. VFF studies were conducted using a "Clearwater VC-1" VFF device. Recoveries after VFF ranged from 55.6% to 102.8%. Immunomagnetic separation was conducted using "Crypto-Scan IMS". Recoveries after IMS ranged from 39.2% to 119.4%. Recoveries for the standard cartridge filter IFA method ranged from 2.5% to 12.5%. Seeded recoveries in surface water after VFF/IMS ranged from 25.2 to 58.8%.
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