• AWWA QTC98337
Provide PDF Format

Learn More

AWWA QTC98337

  • Rapid Detection of Cryptosporidium parvum
  • Conference Proceeding by American Water Works Association, 01/01/1998
  • Publisher: AWWA

$12.00$24.00


This paper presents a simple and rapid screening method for Cryptosporidium parvum, using molecular techniques in a multi-well microplate format. The currently recommended method for C. parvum detection in drinking water cross-reacts with other Cryptosporidium species, has a low recovery efficiency, and is costly and time-consuming. In addition, the drinking water industry still lacks simple, rapid methods for general pathogen detection and identification that are easily amenable to automation. To overcome these problems, a rapid, semi-automated method is being developed that involves nucleic acid labeling during amplification by polymerase chain reaction (PCR). The amplicons are captured in microplates by liquid hybridization to a pre-attached specific probe. The amplicon-probe hybrids are detected by an enzymatic colorimetric reaction. The formation of a purple/brown precipitate within the microplate wells indicates the presence of C. parvum, whereas negative samples remain clear.

Related Products

AWWA JAW6147

AWWA JAW6147

Journal AWWA - Increasing Employee Security and Productivity With Computers ..

$15.00 $30.00

AWWA WQTC52994

AWWA WQTC52994

Optimizing Cryptosporidium Inactivation Credit With Ozone at Las Vegas Using the Integrated Disinfec..

$12.00 $24.00

AWWA ACE61703

AWWA ACE61703

Organic Chloramine Formation in Model Distribution Systems..

$12.00 $24.00

AWWA ACE58129

AWWA ACE58129

The Optimization of MF/UF Membrane Systems on Direct and Clarified Feed Applications - a Real Life E..

$12.00 $24.00