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AWWA QTC98339
- The Next Generation of Cryptosporidium Detection Methods: Two-Colour Fluorescence, "Analysis-Only" Flow Cytometry
- Conference Proceeding by American Water Works Association, 01/01/1998
- Publisher: AWWA
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Routine detection of Cryptosporidium oocysts relies on immunofluorescence assays (IFA) employing fluorescently labeled monoclonal antibodies (mAbs). MAbs used for detection bind non-specifically to detrital particles present in environmental samples resulting in high levels of background fluorescence. A new mAb (Cry104) to Cryptosporidium of the IgG1 subclass exhibited lower levels of non-specific binding to detritus in water samples compared with commercially available antibodies. The specificity of Cry104 has allowed preliminary investigations into two color "analysis-only" flow cytometry by utilizing two selection parameters. Two color flow cytometry results in a significant reduction in fluorescent detrital material being detected following analysis.
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