AWWA QTC98339

Routine detection of Cryptosporidium oocysts relies on immunofluorescence assays (IFA) employing fluorescently labeled monoclonal antibodies (mAbs). MAbs used for detection bind non-specifically to detrital particles present in environmental samples resulting in high levels of background fluorescence. A new mAb (Cry104) to Cryptosporidium of the IgG1 subclass exhibited lower levels of non-specific binding to detritus in water samples compared with commercially available antibodies. The specificity of Cry104 has allowed preliminary investigations into two color "analysis-only" flow cytometry by utilizing two selection parameters. Two color flow cytometry results in a significant reduction in fluorescent detrital material being detected following analysis.

Product Details

Published:

01/01/1998

ISBN(s):

0898679796

Number of Pages:

6

File Size:

1 file , 43 KB