Provide PDF Format
AWWA WQTC52765
- Detect Infectious Cryptosporidium parvum Oocysts Using Integrated Cell Culture-rRNA In Situ Hybridization
- Conference Proceeding by American Water Works Association, 01/01/2000
- Publisher: AWWA
$12.00$24.00
In an infectivity assay, human illeocecal adenocarcinoma (HCT-8) cellswere infected with different concentrations of Cryptosporidium parvum oocysts. After 48 hours of incubation, in-situ hybridization (ISH) using digoxigenin-labeled DNA probes targeted at the C. parvum-specific small subunit rRNA sequences with colorimetric detection was performed to detect infectious oocysts. In addition, immunofluorescencedetection of intracellular reproductive stages of C. parvum using fluorescein-labeled polyclonal antibody against sporozoites of C. parvum was conducted and compared with ISH. Both C. parvumand C. muris were used to infect HCT-8 cells along with ISH to determine the specificity of the DNA probes. Includes 18 references, tables, figures.
Related Products
AWWA C300-97
Reinforced Concrete Pressure Pipe, Steel-Cylinder Type, for Water and Other Liquids..
$23.00 $45.00
AWWA MTC64614
RO Process Design Optimization Based on Stochastic Cost Estimation Model Coupled with Process Perfor..
$12.00 $24.00
AWWA WQTC52875
Low Pressure UV Inactivation of Cryptosporidium parvum and Giardia lamblia Based on Infectivity Assa..
$12.00 $24.00