• AWWA WQTC52827
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AWWA WQTC52827

  • Pitfalls of Applying Clinical Methods for the Detection of Emerging Pathogenic Protozoa to Environmental Water Samples
  • Conference Proceeding by American Water Works Association, 01/01/2000
  • Publisher: AWWA

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The presence of parasitic protozoa in source water used for drinking andagriculture is a growing concern. While there are numerous protocols available for the identification of these organisms in clinical samples (feces, blood and tissues), these methods are not easily nor always successfully translated to environmental water samples. The authors have focused on developing a molecular method to identify Cyclospora cayetanensis in environmental waters. From this work, two important issues that are likely to continue to be troublesome for water microbiologists have been identified. One, well recognized, is the presence of inhibitory substances such as humic acids. Another, less studied, is the presence of non-human pathogenic microorganisms, though genetically similar, in environmental waters. Given that there are pathogenic and non-pathogenic species of both Cryptosporidium and Cyclospora, two of the three most recently emerged waterborne protozoan pathogens, it is more than likely that this difficulty in distinguishing pathogenic and non-pathogenic species will reoccur in the future. Includes 22 references, tables, figures.

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