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AWWA WQTC58785
- Using Ribosome Genesis as a Sensitive Indicator of Microbial Viability to Test Disinfection Efficacy
- Conference Proceeding by American Water Works Association, 11/02/2003
- Publisher: AWWA
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The objective of this study was to demonstrate that ribosomal RNA-targeted techniquescould be used to quantify viable microorganisms when determining disinfection efficacy. Theauthors have developed a new technique to simultaneously measure ribosomal RNA as well asprecursor ribosomal RNA, a key intermediate in the production of ribosomes. Ribosomal RNAand precursor ribosomal RNA were measured simultaneously in cultures of Acinetobactercalcoaceticus<sup>T</sup> and Mycobacterium mucogenicum. The results demonstrate that precursor ribosomalRNA is a sensitive measure of viability. The authors believe this approach holds tremendouspromise for verifying disinfection efficacy for microorganisms using molecular biology-basedmonitoring. In the future, coupling the rapid developments in molecular biology-based technologywith the critical measurement of cellular viability via detection of ribosome genesis may change theway disinfectants are evaluated. Includes 12 references, figures.
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