• AWWA WQTC58791
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AWWA WQTC58791

  • Benchmarking UV Collimated Beam Testing: Inter-laboratory Comparison of the UV Sensitivity of MS2 Coliphage
  • Conference Proceeding by American Water Works Association, 11/02/2003
  • Publisher: AWWA

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As part of the AWWARF Project 2593, Inactivation of Pathogens by Innovative UVTechnologies (PI: Professor James P. Malley), a "Round Robin" ultraviolet (UV) disinfection test wasconducted among the participating laboratories to estimate the level of uncertainty in theperformance of the so-called collimated beam UV test for MS2 coliphage and refine a protocolfor determining the UV sensitivity of microorganisms.The following preparations were conducted prior to testing:a detailed Experimental Protocol was drafted out and vetted by all participants;all radiometers were sent to be calibrated at the same time (in addition, one radiometerwas sent around to each of the other laboratories for direct comparison of readings, andfinally, all radiometers were tested on site using the KI/KIO<sub>3</sub> actinometer (Rahn, 1997)protocol developed by the research team); and,the MS2 stock and E. coli host were prepared in one laboratory (MWD) and shipped toall others by overnight courier.Two Round Robin series were conducted, one in March 2001 and the second in January2003. The results of these tests are presented here and compared among the participatinglaboratories. In addition, the results are compared with the limits set by the National Water Research Institute (NWRI) / American Water Works Association Research Foundation (AWWARF)Guidelines for MS2 testing.list. Includes 7 references, tables, figures, appendix.

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