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AWWA WQTC60597
- Interlaboratory Validation of US EPA Method 1603 (Modified mTEC) for Escherichia coli in Wastewater Effluent
- Conference Proceeding by American Water Works Association, 11/15/2004
- Publisher: AWWA
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The objectives of this slide presentation were to outline information on assessing Method 1603 performance in the matrix of interest; develop quantitative quality control acceptance criteria in a reference matrix to support future assessments of laboratory performance; and, develop quantitative quality control acceptance criteria in the matrix of interest to support future assessments of method performance. The matrices evaluated included: reference matrix (phosphate buffered saline); and, wastewater matrices (disinfected effluent, and secondary effluent). Initial study issues included: eleven of the twelve laboratories reported abnormal growthpatterns on the modified mTEC plates; and,growth restricted to membrane gridlines. Results included: unspiked sample results; overall false positive and false negativeconfirmation rates; spiked PBS results; and, spiked wastewater results. Quantitative QCacceptance criteria included: initial precision and recovery; ongoing precision and recovery; and, MS and MSD.
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