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AWWA WQTC62551
- Use of Microscopic Fluidic Imaging for Identification and Quantification of Organisms in Water
- Conference Proceeding by American Water Works Association, 11/01/2005
- Publisher: AWWA
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The objective of this study was to compare the automated imaging, sizing, and counting produced by theBrightwell DPA4100 Microscopic Fluidic Imaging (MFI) instrument with manual counting byhemocytometer and manual imaging by high-power microscopy for various biological organisms.The MFI instrument operates by drawing the fluid sample through an optical flow cell. Sections of theflowing fluid are illuminated by a pulsed high-intensity source, magnified, imaged on the camera's pixelarray and displayed on the system monitor. The system software identifies, in real time, pixels in the arraythat are wholly or partially contained within a particle image. This information is used to calculateparticle parameters. Samples may be evaluated which contain a wide range of particle types, sizes andconcentrations. MFI software analyzes each particle image both to produce particle size and countdistributions and, to identify and store images of particles meeting user specified criteria. Includes table, figures.
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