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AWWA WQTC62599
- Statistical Model to Assess False Negative PCR Reactions from Water Matrices
- Conference Proceeding by American Water Works Association, 11/01/2005
- Publisher: AWWA
$12.00$24.00
This slide presentation outlines a statistical model for assessing false negative polymerase chain reactions (PCR) from water matrices. Topics covered include: background on the microsporida organism; potential for environmental transmission; recommendation for human testing; molecular methods for microsporidia; experimental design of the AWWARF #2901, ENC-PCR; assay optimization, extraction methods and sample volume; specificity, virtual and actual; calculated efficiency; trend analysis; monitoring inhibitors in matrix; potential use for statistical comparison of IC-Ct; creating a statistical model; source water testing; and, counteracting inhibitors. Includes tables, figures.
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