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AWWA WQTC65727
- In Vitro Assessment of Thyroid and Estrogenic Endocrine Disruptors in WWTPs and Surface Waters in the Greater Paris (France) Area
- Conference Proceeding by American Water Works Association, 11/01/2007
- Publisher: AWWA
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The objective of this study was to evaluate, in parallel, endocrine disruptions of both estrogenic andthyroid hormone peripheral functions generated by complex mixtures of organicmicropollutants.Water samples were collected in two major wastewater treatment plants (WWTPs) of the greater Parisarea. Treatment processes are different where one is based onclassical activated sludge steps (600 000 m<sup>3</sup>/day) and the other on biofilters (240 000 m(sup>3</sup>/day).Samples were taken at the inlet and outlet of the WWTPs and in the river Seine downstream ofthe discharge and at the intake of drinking treatment plants producing water for the Parissuburb.Chemicals were isolated from collected waters by SPE extraction (Waters - OASIScartridges) and were tested using two cellular models for estrogenic (MELN) and thyroiddisruption evaluations. Prior to the assessment of the estrogenic and THs activities, the cytotoxicity of the compoundsfor the cells was investigated using the MTT assay (Mosmann, 1983). Includes 6 references.
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