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AWWA WQTC65752
- Protein Assay in Drinking Water and Reclaimed Water
- Conference Proceeding by American Water Works Association, 11/01/2007
- Publisher: AWWA
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The Lowry and Bradford assays are appropriate for untreated or poorly treated wastewaters, butare not sensitive enough for low protein concentrations such as found in reclaimed waters ordrinking waters. A biomedical assay, NanoOrange® (Molecular Probes Inc., Oregon) which hasbeen used to measure protein content in cells, is very sensitive, but exhibited interferences fromsalts in water, especially from divalent ions. As such it is of limited use for bulk drinking watersamples, but could be used on samples after dialysis pretreatment. For natural organic matter(NOM) isolates and cloud samples, the NanoOrange® method could be used without concern forsalts. The colloidal fraction of natural organic matter (NOM) exhibited the highest protein content based upon theNanoOrange® reagent. Includes 7 references, tables, figures.
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