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AWWA WQTC65824
- Methods for Detecting, Identifying and Quantitating Free-Living Amebae in Water
- Conference Proceeding by American Water Works Association, 11/01/2007
- Publisher: AWWA
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Free-living amebae (FLA) belonging to the genera Naegleria, Acanthamoeba and Hartmanella arefound in diverse water habitats worldwide. Several species of Acanthamoeba and Naegleria fowlerican cause fatal Central Nervous System infections in humans. In the environment, Acanthamoeba,Naegleria and Hartmanella can harbor bacteria including those that have pathogenic potential.Biochemical, immunological, and molecular methods are available to detect, identify, and quantitateFLA in domestic, municipal, and recreational water sources. Small or large volumes of water can becollected, centrifuged or filtered and cultured for growth of amebae on non-nutrient agar coated withEscherichia coli (NNA-E. coli). Several reliable and sensitive conventional polymerase chain reaction(PCR) assays are available for specific identification of amebae. Quantitative real-time PCR assayshave been developed to quantitate the number of amebae present in the sample. Many of the molecularassays are more rapid and sensitive than immunological and biochemical tests. Culture of samples onNNA-E. coli plates prior to PCR allows for detection of live amebae. Includes 40 references, tables, figures.
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