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AWWA WQTC69503
- Contribution to the Optimization of the Detection of Infectious Cryptosporidium by 3D-CC-IFA
- Conference Proceeding by American Water Works Association, 11/01/2008
- Publisher: AWWA
$12.00$24.00
This powerpoint presentation begins by providing a brief overview of Cryptosporidium and a choice of detection methods relative to the objectives. Study objectives were to improve Cryptosporidium risk assessmentby optimizing the CC-IFA method viamaximizing infectious foci countswithin a physiological context, andcombining total and infectious countson the same sample to remove the biasinduced by non-homogeneoussample split. Experimental approach used cell cultures and immunofluorescence assays. Results are given for: method optimization; optimizing stimulation media; optimizing infectious media; IMS dissociation media; direct dual detection andevaluation of oocyst loss; and, impact of reference total count. Conclusions are given for CC-IFA media optimization, and for CC-IFA dual detection. Includes tables, figures.
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